Fig. 2
Confirmation of gata5/6-dependent tmem88a expression in the anterior lateral plate mesoderm. (A,B) RNA sequencing tmem88a expression in RPKM (A) and qPCR tmem88a relative expression (B) for wild-type (black) and gata5/6 morphants (white) at bud and 6-somite (6ss) stages of development. (C,D) Transcript localization of tmem88a and the regional notochord (nt) marker ntl, shown in flatmount in situ hybridization preparations of 6-somite stage control (C) and gata5/6 (D) morphants (dorsal views, anterior to the left). Insets are higher magnifications of the boxed regions. tmem88a expression in the anterior lateral plate mesoderm is outlined (dotted line). n>20 embryos analyzed per panel. *P<0.05, **P<0.01; error bars indicate s.d.