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Fig. S1 Expression of dlx2a and dlx5a in the diencephalon of ascl1a morphants and expression of ascl1a in dlx1a/2a and dlx5a/6a double morphants. Whole mount in situ hybridization using probes for dlx2a (A, B) and dlx5a (C, D). Asterisks indicate prethalamic and hypothalamic reduction of dlx gene expression in 24 and 48 hpf morphant embryos. Tel, telencephalon, Pth, prethalamus, Hy, hypothalamus. Scale bar (A, C)=50 μm; scale bar (B, D)=100 μm.
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Reprinted from Developmental Biology, 381(1), MacDonald, R.B., Pollack, J.N., Debiais-Thibaud, M., Heude, E., Talbot, J.C., and Ekker, M., The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon, 276-85, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.