ZFIN Image: Shih et al., 2013, Fig. S1

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Figure Caption

Fig. S1

Efficiency testing of ddc translational blocking MOs. (A) A 303 bp partial sequence of ddc containing tMO1 or tMO2 targeting site (purple region) was inserted into a pCS2⁺ vector containing CMV promoter (brown region) and GFP (green region) sequences. The binding sequences for ddc tMO1 and tMO2 are shown below the construct map. (B) Embryos were co-injected with designated MO (5 ng) and MO efficiency testing plasmid (100 pg), examined by epifluorescent microscope under bright and dark fields. Representative superimposed images are shown. (C) Percentages of embryos showing GFP in different treatments are shown. Plasmid injected only group, n=106; tMO1,n=103; tMO2, N=64. * p <0.001.

Acknowledgments

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