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Fig. S11
Rarαb1 and rarαb2 mRNA overexpression do not significantly affect RA responsive genes. (A–I) ISH for the RA responsive genes cyp26a1, dhrs3a, and hoxb5b at 8 s. (A, D, G) Control sibling, (B, E, H) rarαb1 mRNA, and (C, F, I) rarαb2 mRNA injected embryos. Injection of either rarαb mRNA did not inhibit RA responsive gene expression. Images in A–C are lateral views with anterior up and dorsal right. Images in D–I are dorsal views with anterior up. (J) qPCR for RA responsive genes cyp26a1, hoxb5a, hoxb8b, dhrs3a at 8 s. (K) Mean CM number from control sibling, rarαb1 mRNA, and rarαb2 mRNA injected Tg(-5.1myl7:DsRed-NLS)f2 embryos. (L) Transfection of HEK 293 cells with DNA for the zebrafish rarαb1 and rarαb2 and pGL3-12XRARE-ef1α:renilla luciferase vector with and without RA treatment. Fold difference in luminescence is indicated in arbitrary units (AU) and reflects the ratio of renilla luciferase (RL) to firefly (FL) luciferase.