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Fig. S2
Dosage curve of an edg5-morpholino antisense oligomer (MO) and its association with cardia bifida. (A) Tg(cmlc2:EGFP, cmlc2:H2AFZmCherry)cy13 embryos injected with different doses of edg5-MO and edg5-5 mm exhibited varying degrees of myocardial migration defects, ranging from Class I (a single heart tube) to Class II (cardiomyocytes either in close proximity or in contact) and Class III (separated cardiomyocytes) at 24 hpf. Scale bar = 100 μm. (B) Percentages of Class I, II and III myocardial migration defects in Tg(cmlc2:EGFP, cmlc2:H2AFZmCherry)cy13 embryos injected with 1–16 ng of edg5-MO at 24 hpf.
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