ZFIN Image: Kim et al., 2013, Fig. S3

Image

Figure Caption

Fig. S3 Liver defects and increased size of hepatocytes in dxa^vu463 mutant zebrafish. (A,B) ORO and DAPI staining of wild type (A) and dxa mutant liver at 6 dpf (B). (C,D) Filipin staining of wild type (C) and dxa liver at 6 dpf (D). Each of single cells are outlined with yellow in A–D, magnified views in lower left corner (C–D) shows absence of cholesterol accumulation in the cytosol of dxa. (E,F) Filipin staining of sibling control (E) and type III dxa (F) for cell size measurement at 8 dpf. Representative cells used for analysis are outlined in blue. (G) Total pixel numbers were measured to compare relative cell size in selected areas in E and F. Thirty cells (10 cells/larvae) were measured and control and mutant size were compared, p<0.001. Scale bar = 50 μm (A,B) and 100 μm (C–F).

Figure Data

Phenotype details

Acknowledgments

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