ZFIN Image: Ninov et al., 2013, Fig. S2

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Figure Caption

Fig. S2

Differentiation of β Cells from Notch Responsive Cells during Juvenile Stages, Related to Figure 2

(A and B) Tg(TP1:CreERT2);Tg(ubi3C:loxp-GFP-loxp:mCherry) larvae were treated with EtOH as vehicle (A) or 4-OHT (B) at 14dpf for 16h, fixed at 17dpf, and stained for 2F11 to mark the IPD cells. 4-OHT treatment resulted in the mosaic labeling of IPD cells (mCherry⁺; 2F11⁺ cells).
(C) Tg(TP1:CreERT2);Tg(insulin:loxP:mCherry-STOP:loxP:H2BGFP) larvae treated with 4-OHT at 15dpf for 24h; the pancreata were analyzed at 18dpf without switching to a high-calorie diet, and after staining with anti-GFP antibodies. No H2BGFP⁺ β-cells were observed in the SIs (19 islets in 30 animals).
(D) Tg(TP1:CreERT2);Tg(insulin:loxP:mCherry-STOP:loxP:H2BGFP) larvae treated with 4-OHT at 2.5dpf for 24h; the pancreata were analyzed at 32dpf. The SIs (arrowheads) are composed of mCherry⁺ β-cells as well as H2BGFP⁺ β-cells. Note that the H2BGFP+ β-cells in the SIs formed groups of several cells, indicating that they underwent amplification after differentiation. Scale bars, 20 μm.

Acknowledgments

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