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Fig. 3
Counterstaining the fli1a:EGFP-expressing pharyngeal arches (a1, a2) with the nucleic acid stain SYTO 59 (red) clarifies the ectomesenchymal phenotypes (double-labeled).
The DV ventral extension differences (arrowheads) are apparent ventral to the red-stained first pharyngeal pouch (p). Compared to the WT (A) this ventral region of mesenchyme is reduced in the edn1 mutant (B) and double mutant (D), and expanded in the hand2 mutant (C). Anterior extension of mesenchyme ventral to the stomodeum (s) is prominent in the hand2 mutant and double mutant (*, C, D).
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