|
Fig. S1
PKD1 expression in 48 hpf zebrafish embryo. A–A3, Whole mount antibody staining for PKD1 was performed in 48 hpf tg(nflk:EGFP) zebrafish embryos. Pictures show whole confocal stacks of several optical sections. GFP signal is shown in green (A), PKD1 staining in red (A2), (A3) displays the merge. Box 1 and 2 mark the area where single optical sections were selected from (D–D2). B, Scheme of a 48 hpf embryo. The red box marks the area of confocal images in (A). C, For background control, embryo was stained without antibody against PKD1. D, Optical sections of the embryo, where colocalization of nuclear GFP signal in endothelial cells and PKD1 expression is shown. E–J, Cross-sections of a 48 hpf tg(fli1:EGFP) embryo. E, Ubiquitous PKD1 expression (red). F, EGFP expression in the vasculature of a tg(fli1:EGFP) embryo, e.g. in the dorsal aorta (DA), posterior cardinal vein (PCV) and dorsal longitudinal anastomotic vessel (DLAV). G, Merge of (E) and (F). H, Confocal image of a 48 hpf tg(fli1:EGFP) zebrafish embryo confirmed co-localization of EGFP and PKD1 in the PCV, DA and DLAV. I, Bright field image. J, Control section lacking the primary PKD1 antibody revealed no staining. Scale bars: 100 μm (A–D2), 25 μm (E–J). ISV: intersomitic vessel. DLAV: dorsal longitudinal anastomotic vessel. PCV: posterior cardinal vein.