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Fig. 2
PKD1 expression silencing resulted in slight vascular defects in tg(fli1:EGFP) zebrafish embryos.
A–A3, Overall morphology of Co-Mo - injected (2 ng) or PKD1 SB-Mo I5 or I6 - injected (500 pg) 48 hpf embryos did not show morphological alterations. Red boxes indicate regions of pictures shown in (B–C3). B–C3, Embryos were analyzed at 48 hpf by confocal microscopy (B–B3) or anti-GFP antibody staining (C–C3). Morpholino-based silencing of PKD1 in zebrafish disrupted partially formation of intersomitic vessels (ISVs) (arrows), the dorsal longitudinal anastomotic vessel (DLAV) (asterisks), and the parachordal lymphangioblasts (PL) (arrowheads) (B–C3). D, Quantification of the number of embryos with vascular defects, ISV defects, DLAV defects and disrupted PL at 48 hpf and 72 hpf. For each group at least 40 embryos were analyzed. White scale bars: 100 μm; black scale bars: 300 μm. *P<0.05, **P<0.01, ***P<0.001.