ZFIN Image: Reimer et al., 2013, Fig. 3

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Figure Caption

Fig. 3 The D4a Receptor Is Necessary to Mediate the Action of Dopamine on Neurogenesis (A) PCR analysis on fluorescence-activated cell-sorted cells from olig2:dsRed/HB9:GFP double-transgenic embryos shows that d4a receptor mRNA is enriched in pMN progenitors, compared to motor neurons. (B) In a dorsal view of double-transgenic embryos, olig2:dsRed⁺/HB9:GFP^- pMN progenitors (brackets) and double-labeled motor neurons are visible. (C) d4a morpholino knockdown is efficient, as shown by PCR. (D) d4a knockdown (MO1) reduces the number of HB9⁺ motor neurons, which cannot be rescued by pergolide (exposure 24–33 hpf). (E) Quantification of motor neurons in (D) (ANOVA, p = 0.0003 with Bonferroni’s posttest, p < 0.01, p < 0.0001). (F) d4a knockdown increases the number of vsx1:GFP⁺ interneurons (p < 0.0001). Error bars represent SEM. Scale bars, 50 μm (B, D, and F). See also Figure S3 and Table S1.

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Acknowledgments

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Reprinted from Developmental Cell, 25(5), Reimer, M.M., Norris, A., Ohnmacht, J., Patani, R., Zhong, Z., Dias, T.B., Kuscha, V., Scott, A.L., Chen, Y.C., Rozov, S., Frazer, S.L., Wyatt, C., Higashijima, S., Patton, E.E., Panula, P., Chandran, S., Becker, T., and Becker, C.G., Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration, 478-491, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell