ZFIN Image: Reimer et al., 2013, Fig. 2

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Figure Caption

Fig. 2 Dopamine Signaling Inversely Affects Motor Neuron and V2 Interneuron Numbers Lateral trunk views at 33 hpf are shown (drug exposure: 24–33 hpf). (A) Treatment with the dopamine agonist pergolide leads to an increase of HB9 immunoreactive motor neurons and a reduction of transgenically labeled presumptive V2 interneurons (vsx1:GFP) in the same embryos. A small subpopulation of double-labeled cells is also strongly reduced (Mann-Whitney U test; p < 0.0001, p < 0.001). (B) NPA, another dopamine agonist, increases the number of HB9:GFP⁺ motor neurons and decreases the number of vsx1:GFP⁺ cells (left column). The D4 antagonist L-745870 has the inverse effect on numbers of these cell types (right column). Error bars represent SEM. Scale bars, 50 μm. See also Figure S2.

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Acknowledgments

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Reprinted from Developmental Cell, 25(5), Reimer, M.M., Norris, A., Ohnmacht, J., Patani, R., Zhong, Z., Dias, T.B., Kuscha, V., Scott, A.L., Chen, Y.C., Rozov, S., Frazer, S.L., Wyatt, C., Higashijima, S., Patton, E.E., Panula, P., Chandran, S., Becker, T., and Becker, C.G., Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration, 478-491, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell