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Fig. S2 Validation of the miR-30 morpholino. (A) Injection of zebrafish embryos with GFP fused to a 3′UTR containing (1) tandem miR-30 perfect target sites (GFP-PTS). (2) Co-injection of miR-30 RNA with the GFP-PTS reporter mRNA. (3) Co-injection of miR-30 RNA and the miR-30 morpholino with the GFP-PTS reporter. (B) Western blot of embryos as in 1–3 with antibodies against GFP and α-tubulin as a loading control. (C) Histogram to quantify the restoration of GFP protein following miR-30 morpholino coinjection. GFP levels are normalised against α-tubulin and presented as a percentage of the GFP-PTS injected embryos.
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