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Fig. S1 (related to Fig. 1) TH1+ neurons influence the generation of late born motor neurons. A: Exposing embryos to EdU at the given time points leads to different labeling frequencies of islet-1:GFP+ and HB9+ neurons in spinal cross sections at 72 hpf. Over 40% of HB9+ motor neurons and over 70% of islet-1:GFP+ motor neurons are still born after 24 hpf (arrows indicate double labeled cells). Almost all motor neurons are born by 51 hpf. At least 6 larvae were analyzed per time point. B: Th genes are detectably expressed only in the brain. Expression of th1 and th2 mRNA is detectable in the head (arrows) and cranial ganglia (arrowhead), but not in the spinal cord (position indicated by brackets) by in situ hybridization (lateral views) at 1 and 2 days post fertilization (dpf). C: 6-OHDA reduces the number of TH1+ diencephalic neurons (dorsal head views, rostral is up) and attenuates islet-1:GFP+ motor neuron development. This is fully rescued by NPA (exposure 24 to 56 hpf, ANOVA, ***P < 0.0001). D, E: Injection of a morpholino against th1 leads to a significant reduction in the number of HB9:GFP+ motor neurons (D; lateral trunk views; 33 hpf; ***P = 0.001), and attenuation of islet-1:GFP+ motor neuron development (E), which is fully rescued by NPA application (drug exposure 24 to 56 hpf; ANOVA, ***P < 0.0001). Error bars represent SEM. Scale bars: A: 60 μm, B: 100 μm, C: 100μm, D: 50 μm.
Reprinted from Developmental Cell, 25(5), Reimer, M.M., Norris, A., Ohnmacht, J., Patani, R., Zhong, Z., Dias, T.B., Kuscha, V., Scott, A.L., Chen, Y.C., Rozov, S., Frazer, S.L., Wyatt, C., Higashijima, S., Patton, E.E., Panula, P., Chandran, S., Becker, T., and Becker, C.G., Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration, 478-491, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell