ZFIN Image: Albacker et al., 2013, Fig. 3

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Figure Caption

Fig. 3

SUV39H1 overexpression does not impact cell cycle or muscle differentiation status of mature tumors.

(A) Experimental design for in vivo cell cycle analysis. One-cell stage embryos were injected with three constructs rag2-hKRAS^G12D, either rag2-mCherry or rag2-hSUV39H1, and mylz2-GFP. High-GFP expressing fish were raised to 30 dpf, and those with tumors were injected IP with EdU. After 24 hours, the injected fish were sacrificed and cryopreserved. (B) Number of EdU-positive cells, stained on GFP-positive tumor sections, was normalized to number of total cells, determined by presence of DAPI. No difference was observed between the control and SUV39H1 tumors (n = 5 for each group; p = 0.78). (C) H&E staining of RMS tumors overexpression either mCherry or SUV39H1. Both sets of tumors are very poorly differentiated; they also look similar to each other, indicating no difference in differentiation state of the tumors (bars represent 50 um, n = 6 for each group).

Acknowledgments

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