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Fig. 5 The Tg(LCRRH2-RH2-2:GFP)pt115-j line expresses GFP in several types of photoreceptors, the hatching gland, pineal gland, and olfactory bulb. (A) Bright field and fluorescence images show GFP signals (green) in the hatching grand, pineal gland, and a small region in the forebrain (arrowhead) at 3 dpf. (B) Imaging on the frontal plane revealed that the GFP-positive cells in the forebrain at 48 hpf (panel A, arrowhead) correspond to a small region in the olfactory bulb (arrows), immediately next to the olfactory epithelia (OE). The anterior is on top. (C) A more ventral-positioned imaging on the frontal plane at 48 hpf (compared to panel B) revealed extensive GFP expression in the mesenchyme-derived tissues in the head and trunk (arrows). (D and E) Vertical (D) and transverse (E) confocal imaging of photoreceptors in 8-mpf adult retina revealed GFP signals in the green, red, and blue cones as well as some rods (arrowheads). However, certain rods do not express GFP (asterisks). Double cones were labeled with zpr1 antibody in red; arrow G, green cones; arrow B, blue cones; arrow R, red cones. (F and G) GFP is expressed in the photoreceptor layer in the entire retina at 1 mpf. (H and I) At 9 mpf, strong GFP expression is maintained in the photoreceptor layer in the entire retina. A-P and D-V arrows indicate anterior–posterior and dorsal–ventral directions, respectively.
Reprinted from Gene expression patterns : GEP, 13(5-6), Fang, W., Bonaffini, S., Zou, J., Wang, X., Zhang, C., Tsujimura, T., Kawamura, S., and Wei, X., Characterization of transgenic zebrafish lines that express GFP in the retina, pineal gland, olfactory bulb, hatching gland, and optic tectum, 150-9, Copyright (2013) with permission from Elsevier. Full text @ Gene Expr. Patterns