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Fig. 6 RA-FGF Antagonism Is Required to Generate the Forelimb Field (A) Assessment of forelimb field Tbx5 expression (f) in control versus FGF8-treated wild-type (WT) embryos cultured in vitro for 12 hr. Heart Tbx5 mRNA (h) and somite Uncx4.1 mRNA (s) are also indicated. (B) Rescue of missing pectoral fins in raldh2 (nls) mutant zebrafish by crossing to heat-shock-inducible dn-fgfr1 transgenic fish (heat shock at 37°C; 8–10 hpf). Arrows indicate pectoral fins. See also Figure S6.
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