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Figure Caption
Fig. S2 Successful knockdown of miR-34b by morpholinos. (A) Two morpholinos were designed and shown to block the biogenesis of mature miR-34b (WISH). (B) Image of Tg(cmyb:EGFP) embryo shows that MCCs accumulate in the middle part of pronephric duct at 3 dpf after miR-34b MO2 injection. (C) Manual count of MCCs labeled by Tg(cmyb:EGFP) at 36 and 48 hpf. n=30 for Ctrl MO and miR-34b MO (mean ± s.d.). (D) Representative results show hematopoiesis after miR-34b knockdown.
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