Fig. S4

Fig. S4 Expression of macc1, thsz2, pkdcc (LOC565254), vlk, lix1l, and pcdh19 at 24 and 48hrs of zebrafish development. All images are lateral views, anterior to the left. A, C, E, G, I and K are 24 hpf and B, D, F, H, J and L are 48hpf. A, B) macc1 expression at 24hpf is observed in the olfactory bulb, eye, midbrainhindbrain boundary, otic vesicle, and pronephros. At 48hpf, macc1 expression continues in the otic vesicle and becomes apparent in the roof of the stomodeum. C, D) thsz2 is expressed in the olfactory bulb, eye and hindbrain at both 24 and 48hpf. Weak expression also occurs in the branchial arches. E, F) pkdcc (LOC565254) is expressed diffusely in the eye and anterior and posterior mesoderm at 24hpf and resolves to the eye, branchial arches, and the region of the forming neurocranium at 48hpf. G, H) vlk expression at 24hpf is observed in the eye, posterior branchial arches and fin bud. By 48hpf, expression expands to the more anterior branchial arches. I, J) Expression of lix1l is observed in the eye and hindbrain at 24 and 48hpf as well as more diffusely in the branchial arch region at 48hpf. K, L) pcdh19 is expressed in all CNS structures at 24hpf and resolves to the olfactory bulb, eye, midbrain, and hindbrain at 48hpf. Abbreviations: b, brain; ba, branchial arches; e, eye; fb, fin bud; hb, hindbrain; m, mesoderm; mb, midbrain; mhb midbrain-hindbrain boundary; n, neurocranium; o, olfactory bulb; ov, otic vesicle; pba, posterior branchial arches; pn, pronephros; s, stomodeum; sc, spinal cord.