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Fig. 4 PD98059 and TPCK selectively suppress different downstream RAS signaling pathways in zebrafish embryos. Western blot analysis was performed using Tg(hsp70-HRASG12V) embryos to study the phosphorylation status of Erk1/2 (T202/Y204), Akt (S473) and Rps6 (S240). The embryos were treated with PD98059 (18.7 μM), TPCK (1 μM) or DMSO control from 22 hpf, heat shocked from 24-25 hpf at 37°C and whole embryos were homogenized in 1× SDS sample buffer at 28 hpf.
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