Fig. 2

Fig. 2 A small-molecule screen in Tg(hsp70-HRAS^G12V) zebrafish embryos. (A) Scheme of the chemical screen. Heterozygous Tg(hsp70-HRAS^G12V) embryos were placed in a 48-well plate for chemical treatment starting at 22 hpf and heat shocked from 24-25 hpf in a 37°C waterbath to activate RAS signaling (dashed line). At 30 hpf, embryos were fixed and the dusp6 expression level was evaluated by ISH. The solid line represents the dynamic changes in dusp6 RNA level, as confirmed by RT-PCR; the dashed line represents the predicted activation of RAS based on hsp70 promoter dynamics (Le et al., 2007). (B) ISH of dusp6 on embryos treated with PD98059, TPCK, Lovastatin, Tyrphostin A9, Valinomycin and Catechin.