|
Fig. S1 Morphology of gata5 MO1 (20 ng/embryo), gata5 MO2 (16 ng/embryo), otx2 MO1 (1.2 ng/embryo), and otx2 MO2 (1.2 ng/embryo) injected embryos at 3 dpf. (A) Selected image of control MO injected embryo which represents 21 out of 22 of control morphants. (B) Selected image of gata5 MO1 injected embryo which represents 43 out of the 61 gata5 MO1 morphants exhibited pericardial swollen and unlooped heart. (C) Selected gata5 MO2 injected embryo which represents 57 out of the 69 gata5 MO2 morphants exhibited similar heart defects. (D) Selected image of otx2 MO1 injected embryo which represents 27 out of the 58 otx2 MO1 morphants with mild defect as exhibited pericardial swollen, unlooped heart and almost normal body length. (E) Selected image represent 14 out of the 58 otx2 MO1 morphants exhibited more sever defect as pericardial swollen, unlooped heart and body length shorten. (F) Selected image of otx2 MO2 injected embryo which represents 35 out of 62 otx2 MO2 morphants with mild defect. (G) Selected image represent 23 out of the 62 otx2 MO2 morphants exhibited more server defect.
Reprinted from Developmental Biology, 357(2), Tseng, W.F., Jang, T.H., Huang, C.B., and Yuh, C.H., An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish, 541-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.