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Fig. S1 In situ hybridization analysis for etv2, ephrin B2a, and ephB4. (A,B) Etv2 expression in wild type embryos at 4 somite (A) and 8 somite stages (B). Note that etv2 expression is observed in the medial angioblasts (arrowheads) only. Flat mounted embryos, dorsal view, A – anterior; P – posterior. (C-F) Arterial expression of ephrin B2a (C,E) and venous expression of ephb4a (D,F) is absent in etv2y11-/- mutant (E) or morphant (F) embryos at 24-27 hpf. Arrows in (C) indicate expression in the dorsal aorta. Arrowheads in (D) indicate expression in the posterior cardinal vein.
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Reprinted from Developmental Cell, 25(2), Kohli, V., Schumacher, J.A., Desai, S.P., Rehn, K., and Sumanas, S., Arterial and venous progenitors of the major axial vessels originate at distinct locations, 196-206, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell