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Fig. 4 Knock-down of alk does not affect proliferation but induces apoptosis in the hindbrain.
(A–C) Confocal sections of 22 hpf embryos after immunostaining with pH 3 (green) and TUNEL (red). Compared to WT (A), both alk ATG-MO injected (B) or alk Spl-MO injected (C) embryos show pH 3 positive cells at normal positions, but more TUNEL positive cells are evident. Note that the size of neural tube in (B,C) is smaller than in (A). (D) Y-axis indicates numbers of pH 3 positive cells in a 50 µm thick confocal stack of hindbrain. Numbers are not significantly different in alk ATG-MO or alk Spl-MO from WT. (E) Y-axis indicates numbers of TUNEL positive cells in the same samples. Both alk ATG-MO and alk Spl-MO numbers were different from that in WT with high significance (***p<0.001). Mean ± SEM, n = 5 embryos in each group. Unpaired two tailed t-test. Scale bars: 50 μm.