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Fig. 4 Electroretinogram recordings of mglur6b-depleted zebrafish larvae.
The downregulation of mGluR6b leads to a dose dependent decrease of the ERG b-wave in 5 day old zebrafish larvae indicating a diminished ON-response. A: Plotted b-wave amplitudes at different light intensities. Control Morpholino (MO) injected larvae of different concentrations showed no significant differences in b-wave amplitude, neither among each other nor in comparison to uninjected wild type larvae (data not shown). Therefore, recordings of all control MO larvae (n = 46) were taken together to build one curve. Injection of mglur6b MO leads to a dose dependent depletion of the b-wave (1.5 ng mglur6b MO: n = 14; 2.99 ng MO: n = 24; 7.48 ng MO: n = 13). All data points represent the means ± SEM. B: Significance of the b-wave amplitude reduction was calculated using a 2-way ANOVA (* = p<0.1; ** = p<0.01; *** = p<0.001).