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Fig. 7 Eaf1/2 activity is evolutionarily conserved from zebrafish to human. (A) Phenotypes of zebrafish embryos injected with human EAF1 (hEaf1) or EAF2 (hEaf2) mRNA at 72 hpf. (B) Morphology of zebrafish embryos injected with human EAF1 or EAF2 mRNA at 3 dpf (200-500 pg/embryo) was scored as normal (white), moderately defective (gray) or severely defective (black); embryos injected with GFP mRNA (200-500 pg/embryo) were used as a control. (C) six3b displayed enhanced expression in zebrafish embryos in which human EAF1 or EAF2 was ectopically expressed. (D) 8xTopFlash activity enhanced by human β-catenin was suppressed in the HEK 293 cell line by human EAF1 or EAF2. (E) Downregulation of c-MYC promoter activity by human full-length EAF1 or EAF2. Mean±s.d.; **P<0.01, *P<0.05 (Student’s t-test). (F) Co-immunoprecipitation of human EAF1 or EAF2 with full-length human β-catenin. (G) Human EAF1 and EAF2 interact with components of the human β-catenin transcriptional complex, including TCF4, c-JUN and AXIN, in vivo. Ca-c, dorsal views, anterior to the left; Cd-f, lateral views, anterior to the left. WCL, whole cell lysate.