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Fig. S7 VP16 and EnR are not functional in vivo and Eaf1/2 act as transcriptional repressors. (A,B) Six3b staining indicated normal patterning of anterior neural ectoderm in embryos injected with mRNA of the engrailed domain (EnR) or the VP16 domain (A), and in embryos injected with EnR or VP16 fused with the C-terminus of Eaf1 or Eaf2 (B). (C) In the embryos injected with eaf1/2-EnR or eaf1/2-N-EnR mRNA, the expression of the dorsal marker genes gsc and chd displayed similarly expanded patterns. (A,B) Dorsal views, anterior to the left; (C) animal views, dorsal to the right. All mRNA injections were 50-100 pg/embryo.