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Fig. 3 Time-dependent expression of HCV core and gfp during the early larva stage.
A. Transcription level of core and gfp in pFL-GIC injected zebrafish larvae at 3-, 6- and 9-dpf was examined by RT-PCR. β-actin was used as a loading control. The band semiquantitative density scanning was done and normalized by β-actin signal for their transcriptional level evaluation (the right histogram. * p<0.05, ** p<0.01). B. Translation level of CORE and GFP in pFL-GIC injected larvae was detected with Western blotting at 3-, 6- and 9-dpf. β-ACTIN was used as a loading control. The bands of CORE and GFP were scanned and normalized by β-ACTIN signal for their protein level evaluation (the right histogram. * p<0.05).