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Fig. S7
Low dose injection (50 pg) of HOXC9 mRNA shows no effect on zebrafish vascular morphology.
(A,D) Overall morphology of 48 hpf (A) and 72 hpf (D) zebrafish embryos after injection of control morpholino. Red box shows region displayed in (B,C,E,F). (B,E) Normal formation of the PLs (arrows) in 48 hpf (B) and 72 hpf (E) tg(fli1:EGFP) fish embryos after injection of 4 ng control morpholino or 4 ng control morpholino combined with 50 pg HOXC9 mRNA (C,F). (G) RT-PCR analysis of zebrafish lysates injected with indicated morpholinos showed no regulation of stabilin 1 by HOXC9 silencing. (H) RT-PCR analysis of zebrafish lysates injected with indicated mRNA showed no regulation of stabilin 1 by HOXC9 overexpression. Black scale bar: 500 μm. White scale bar: 50 μm.