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Fig. 3 Effect of cas, vox and pou2 overexpression on endoderm formation. (A-E,G-J) Zebrafish embryos were either left uninjected (A,G) or injected with the RNA indicated bottom right (B-E,H-J), then processed for in situ hybridization with a sox17 probe. (F,K) qRT-PCR analysis. Embryos were injected with the indicated RNA(s). RNA was then extracted and processed for qRT-PCR analysis of sox17. Levels relative to uninjected controls (WT, set at 1) are plotted. For statistical analyses, samples cas+vox or cas+ved or cas+vent are compared with cas (F) or cas+pou2+vox with cas+pou2 (K); ***P<0.005; error bars indicate s.d.