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Fig. 2 In vitro binding assay between Cas, Pou2 and Vox. (A-C,E-G) Co-IP experiments. The RNAs indicated below each panel were translated in vitro in the presence (untagged proteins) or absence (myc- or HA-tagged proteins) of radiolabeled methionine, except for C, where tagged protein was also radiolabeled, then processed for IP with the relevant antibody and resolved by SDS-PAGE. The expected position of the tested proteins is indicated on the left of each panel. Note that Vox and Pou2 artifactually migrate as a doublet in A,B,G. (D) Structure of the vox RNA variant open reading frames used for translation, illustrating the deletions in the ΔN-Vox and ΔC-Vox variants.