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Fig. s2 (A) Embryos co-injected with 5? dner-EGFP cRNA and control morpholino showed strong EGFP expression (upper panel). In contrast, the EGFP signal was abolished in embryos co-injected with 5? dner-EGFP cRNA and dner MO1 (lower panel). (B) The levels of GFP expression were confirmed by Western blot analysis and quantified. (C) The efficacy of MO2 was validated by RT-PCR. Wild-type dner mRNA produced a 2235-bp PCR product, while alternatively spliced transcripts from morphant embryos yielded a 1988-bp fragment.
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Reprinted from Developmental Biology, 375(1), Hsieh, F.Y., Ma, T.L., Shih, H.Y., Lin, S.J., Huang, C.W., Wang, H.Y., and Cheng, Y.C., Dner inhibits neural progenitor proliferation and induces neuronal and glial differentiation in zebrafish, 1-12, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.