ZFIN Image: Djiotsa et al., 2012, Fig. S5

Image

Figure Caption

Fig. S5 Expression of the Tg(ARX enhancer:GFP) transgene is increased by arx knock-down. Representative images of Tg(ARX_enhancer:GFP) transgenic embryos that have been injected with arx morpholino in comparison to uninjected embryos and embryos injected with a standard control morpholino. Fluorescent overview images were taken with an inverted microscope (A), while fluorescent intensities were measured by confocal microscopy (B) and quantified (C) as described in Materials and Method. A. Lateral view of 2 dpf Tg(ARX_enhancer:GFP) embryos injected with ARX morpholino (2ng), control morpholino (2ng) or uninjected. B. Maximum intensity projection of confocal stack of 3 dpf Tg(ARX_enhancer:GFP) embryos (dorsal view) injected with ARX morpholino (2ng), control morpholino (2ng) or uninjected. C. Fluorescent intensity measurements. Means of 10 embryos ± Standard Deviation. Different from control at *P < 0.001.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.