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Fig. s5 Depletion of Pax6.2 by morpholino knock-down. A) a Pax6.2 morpholino. and B) a control morpholino were Injected into zebrafish oocytes. Pools of embryos were fixed at 2 dpf and tested for the presence of Pax6.2 transcript by in situ hybridization. Pax6.2 ISH signal was absent or greatly reduced in the majority of pax6.2 morpholino injected embryos, but was unaffected by control morpholino injections. C, D) Diameter of the eye was measured relative to the total body size of the embryos, and the average ratio of eye diameter versus body length was calculated. E) Chart showing the eye diameter of pax6.2 morphants was on average 30% reduced compared to embryos injected with a non-specific morpholino.