ZFIN Image: Ma et al., 2012, Fig. S4

Image

Figure Caption

Fig. S4 Genes expression in zebrafish cloche mutant, foxn1 morphants, mouse thymus, and TEC cell lines. (A) The expression of six genes (foxn1, rag1, cbf&betal, cdca7, mcm2, and runx3) in zebrafish cloche mutants by WISH. Anterior to the left and dorsal to the up; circles mark the thymus. (B) Transverse sectioning after WISH showed that the expression of cbfβ, cdca7, mcm2, and runx3 in the thymus was reduced in foxn1 morphants compared with controls. Dashed lines mark the thymus. (C) The expression of mcm2, cdca7, runx3, cbfβ, and actin in mouse thymus and TEC cell lines (m4c18 and m1c6) using semiquantitative RT-PCR.

Acknowledgments

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