ZFIN Image: Dougherty et al., 2013, Fig. S2

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Figure Caption

Fig. S2 Characterization of sox10:irf6^R84C transgenics. (A) Transgene contains mutant irf6 (R84C, R84H, or R84K) under the control of the sox10 promoter, followed by a fluorescent tag (cmlc2:egfp) to facilitate screening. (B,C) Expression pattern of exogenous irf6 matches that of sox10 at 10 somites. (D,E) TUNEL assay for apoptosis confirms that clefting in sox10:irf6^R84C is not a result of cell death. (F,G,J,K) Expression analysis of neural crest markers dlx2a and sox9a show that early neural crest development is not significantly affected in the irf6 mutant. (F,J) At 24 hpf, the expression pattern of dlx2a is unaffected in the developing pharyngeal arches. (G,K) Similarly, pharyngeal arch expression of sox9a is similar in wild-type and mutant embryos. (H,I,L,M) By 55 hpf, although the head is already noticeably smaller in mutants, patterning of sox9a is similar, highlighting the developing ethmoid plate, mandible, ceratohyal and ceratobranchials.

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