ZFIN Image: Garnaas et al., 2012, Fig. s1

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Figure Caption

Fig. s1 A chemical genetic screen identifies retinoic acid as a regulator of embryonic zebrafish liver development. A chemical genetic screen identifies retinoic acid as a regulator of embryonic zebrafish liver development. (A) Chemical genetic screen workflow. Wild type (WT) or lfabp:GFP transgenic reporter fish were exposed to individual compounds from a library of 2640 known bioactives over the course of embryonic liver development (18-72 hpf). Alterations in liver size were detected by fluorescence microscopy and in situ hybridization for lfabp. (B) Eight hits corresponding to six compounds were identified in the screen. Retinoic acid, isotretinoin, retinol, and beta-carotene increased liver size, whereas RA pathway inhibitors diethylaminobenzaldehyde (DEAB) and disulfiram decreased liver size.

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Reprinted from Developmental Biology, 372(2), Garnaas, M.K., Cutting, C.C., Meyers, A., Kelsey, P.B., Harris, J.M., North, T.E., and Goessling, W., Rargb regulates organ laterality in a zebrafish model of right atrial isomerism, 178-189, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.