ZFIN Image: Chu et al., 2012, Fig. 4

Image

Figure Caption

Fig. 4 Rock1, but not Rock2a, is necessary for YSL formation in the zebrafish embryo.>

(A) Protein domains of Rock1 and Rock2a and the putative dominant-negative (dn) construct. (B) Schematic of single-cell injection into the 2-cell stage embryo. (C) Embryos at oblong stage were stained for β-tubulin (white), F-actin (red) and DNA (blue). Images are projections of multiple focal planes. Arrowheads in Ci′,i′′ indicate a mitotic cell in late anaphase. Injection of dn-Rock2a (Cii–ii′′) did not affect YSL formation. Injection of dn-Rock1 (Ciii–iii′′) caused expansion of the YSL. (D) Statistical data for embryos expressing dn-Rock1 showing dose-dependent YSL expansion.

Acknowledgments

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