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Fig. 5 Robust reconstruction and segmentation of cells in the presomitic mesoderm.
(A-C) Raw image data showing presomitic mesoderm on 2D image planes (XY,YZ, and XZ) at 3ss. (D-F) Segmentation meshes overlaid on reconstructed membrane images demonstrate excellent localization. Each mesh was randomly colored for visually separating adjacent cells easily. (G,H) 3D rendering of membrane segmentations at 3ss and 5ss. Somites 3, 4 and 5 at 5ss are formed from the presomitic tissue at 3ss by cell sorting and rearrangement.
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