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Figure Caption
Fig. 2 Regeneration of the myocardial layer and induction of Gata4 expression in young and old fish hearts.
(A-F′) MHC staining of regenerating hearts at 7 dpa (A–B′), 14 dpa (C–D′) and 30 dpa (E-F′) in young (A,A′,C,C′,E,E′) and old (B,B′,D,D′,F,F′) fish. A′-F′ show higher magnification images of the boxed areas in A-F. Open arrowheads indicate MHC signals in the regenerating area. For simplicity, not all signals are pointed. Scale bar: 50 μm; the degree of zoom: ×5.5. (G,H) Gata4 immunoreactivity was not detected immediately after amputation. H shows a close up of the boxed areas in G. Scale bar: 50 μm; the degree of zoom: ×2. (I-J′) Gata4 staining of regenerating hearts at 7 dpa in young (I,I′) and old (J,J′) fish. Black arrowheads indicate Gata4 signals. I′ and J′ show close ups of the boxed areas in I and J. Sections were counterstained with hematoxylin. Dotted lines indicate the amputated planes of 7 and 14 dpa hearts. Brackets indicate the regenerating areas of 30 dpa hearts. Scale bar: 50μm; the degree of zoom: ×2.
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