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Fig. 3 hsp90a mRNA is up-regulated in unc45b-/- mutant embryos at 48 hpf.
In situ hybridization with anti-sense probes for hsp90a.1 (a,d,g,j), hsp90a.2 (b,e,h,k), and hsp90ab.1 (c,f,i,l) mRNA transcripts was performed on wild type siblings (a–c); unc45a-/- (d–f); unc45b-/- (g–i); and unc45b-/-; unc45a-/- (j–l) mutants. unc45b-/- and unc45b-/-; unc45a-/- mutants displayed increased expression of hsp90a.1 (g,j) and hsp90a.2 (h,k), but not hsp90ab.1.
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