Fig. 4

Fig. 4 Target protectors relieve miR-1/206-mediated repression of the vegfaa 32UTR. (A) Sequence of each target site, the cognate target protector (TP, violet) and miR-1. (B) Luciferase assay to test the action of vegfaa TP as described in Fig. 2. (C) Luciferase reporter assay to determine the ability of the TPs to prevent miR-1/206-mediated repression of the luciferase-vegfaa 3′UTR. Repression of the luciferase-vegfaa 3′UTR is relieved in the presence of vegfaa TPs (+TP). (D) Confocal analysis of GFP expression in the ISV of Tg(fli-nGFP)^y7 zebrafish embryos injected with control MO or vegf-TP^miR-1 at the one-cell stage. (E) Quantitation of the number of nuclei per ISV of control MO-, miR-1/206 MO- or TP-injected embryos. Note the increase in endothelial cells in the ISV of embryos injected with TP against the miR-1/206 target sites in the vegfaa 3′UTR. (C,E) Mean ± s.d.; Student′s t-test.