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Fig. 4 Cxcr4 function is required for heart regeneration. (A-F′) MHC (brown) and hematoxylin (purple) staining of control (A,A′,C,C′,E,E′) and CXCR4-antagonist-treated (B,B′,D,D′,F,F′) heart at 14 dpa (A-B′), 30 dpa (C-D′) and 60 dpa (E-F′). (G-L′) MHC and hematoxylin staining of wild-type (G,G′,I,I′,K,K′) and cxcr4b-/- (H,H′,J,J′,L,L′) heart at 14 dpa (G-H′), 30 dpa (I-J′) and 60 dpa (K-L′). A′-L′ show close up of the boxed area in A-L, respectively. The arrowheads in A′, B′, G′ and H′ point to the MHC signal in the regenerating area. The black and red arrows in C′-F′ and I′-L′ point to the presence and absence of a sealed muscle wall, respectively. Dotted lines in A, B, G and H indicate the amputation planes. Scale bars: 50 μm.