ZFIN Image: Jensen et al., 2012, Fig. S2

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Figure Caption

Fig. S2 Morphology and Expression Patterns of Sonic Hedgehog and Acetylated Tubulin in Zebrafish Embryos, Related to Figures 1 and 2 Zebrafish embryos at the 1–2 cell stage received injections of specific morpholinos targeting Bmal1 (B1MO), or Period2 (P2MO) (0.2 pmol/embryo) and subsequently raised under LL or LD conditions. Scrambled morpholino (scrMO, 0.2 pmol/embryo), 5 base–pair mismatch bmal1 (B1misMO, 0.2 pmol/embryo), and 5 base–pair mismatch period2 (P2misMO, 0.2 pmol/embryo) were used as controls. Expression of sonic hedgehog (shh) at 24 or 48 hpf was detected by in situ hybridization. Expression of acetylated tubulin (Ac–Tub) was detected by immunohistochemistry using a specific anti Ac–Tub antibody. NC = notochord; EN = enteric nerve.

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