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Fig. S2
Cxcr7b localization to the plasma membrane is not enhanced by β-arrestin knockdown. Wild-type embryos were co-injected at the 1-cell stage with mRNAs for Cxcr7b-EGFP-globin32UTR and mCherry-F-globin32UTR as well as with MO mixes containing either control MO (COMO) or β-arrestin MOs. Confocal images (63×) were acquired at (A) 50% epiboly (5.5. hpf) and (B) 80% epiboly (8 hpf). The graph shows the quantification of the pixel intensity of Cxcr7b-EGFP located on the cell membrane at 5.5 hpf. The average pixel intensity in the control is set to 1. N, number of cells analyzed; **P<0.05, Student’s t-test.
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