ZFIN Image: Zeng et al., 2012, Fig. S3

Image

Figure Caption

Fig. S3

Loss of Klf5 by splice morpholino shows heart and gut phenotypes.

(AD) Hydrocephalus (arrowhead) is observed at 48 hpf in klf5a^{e3i3 MO} morphants (B) as compared with WT embryos (A). klf5a^e3i3 morphants show malformation of the gut at 96 hpf (arrow, D) as compared with WT embryos (C). The magnification for AD is shown by the scale bar in D. (E) Amplification of klf5a cDNA with primers flanking the exon 3 shows a reduction of the transcript in klf5a^e3i3 morphant. ef1α serves as a loading control. (F) qPCR expression analysis of bmp4, shha and ihha in klf5a morphants shows no significant change when compared with WT at 96 hpf in klf5a^e3i3 morphants.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 367(2), Zeng, L., and Childs, S.J., The smooth muscle microRNA miR-145 regulates gut epithelial development via a paracrine mechanism, 178-186, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.