|
Fig. S4 (A-E) WISH analyses of mpx in the CHT region. Zebrafish embryos treated with DMSO or 300 nM SCH79797 from 12 hpf to 36 hpf, or injected with 4 ng Ctrl MO, 4 ng F2r MO1 or 4 ng F2r MO2, and stained with riboprobes to mpx at 48 hpf. Embryos were lateral views with anterior to the left. CHT, caudal hematopoietic tissue. (F-J) WISH analyses of rag1 in the thymus. Zebrafish embryos treated with DMSO or 100 nM SCH79797 from 12 hpf to 36 hpf, or injected with 4 ng Ctrl MO, 4 ng F2r MO1 or 4 ng F2r MO2, and stained with riboprobes to rag1 at 4 dpf. (K-P) Confocal microscopy analyses of gata1+lmo2+ EMPs. Tg(gata1:EGFP; lmo2:DsRed) double transgenic embryos were injected with 4 ng Ctrl MO or 4 ng F2r MO1, followed by multi-layer confocal scanning and z-stack processing at 30 hpf. PBI, posterior blood island. (Q) Statistical analysis of the confocal data (n=5).
Reprinted from Developmental Cell, 22(5), Yue, R., Li, H., Liu, H., Li, Y., Wei, B., Gao, G., Jin, Y., Liu, T., Wei, L., Du, J., and Pei, G., Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition, 1092-1100, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell