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Fig. S9 The injury-induced response of the epicardium and endocardium is not affected by Alk5/4-i treatment. (A-C) Immunofluorescent analysis of heart sections using the cardiac marker Tropomyosin (TPM, blue in left panels and red in middle and right panels) and Raldh2 (green). DAPI (yellow) in left panels labels all nuclei. (A′-C′) Higher magnification of the framed area in the left panel. (A,A′) In uninjured hearts, the epicardium is demarcated by Raldh2 expression (arrows). (B,B2) At 14 dpci, control hearts display the activation of Raldh2 expression in the endocardium (arrowheads). Epicardial cells surround the injured area (arrows). Raldh2-positive cells invade the post-infarct area (dashed line). (C,C2) At 14 dpci, Alk5/4-i-treated hearts show a similar Raldh2 expression pattern to the control. Scale bar: 300 μm.