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Fig. 3
Terminal differentiation of intestinal goblet cells is affected in agr2 morphants.
Significant increases in immature Alcian blue-stained goblet cell numbers were detected in 104- (C, n = 46) and 120-hpf (G, n = 23) agr2 morphants compared to those in either 104- (A, n = 40) and 120-hpf (E, n = 30) wild type or in 104- (B, n = 41) and 120-hpf (F, n = 27) agr2–5 mmMO1 and 5 mmMO2-coinjected embryos. Inset shows a mature and an immature goblet cell. Comparison of both immature and mature goblet cell numbers among agr2 morphants, wild type or agr2–5 mmMO1 and 5 mmMO2-coinjected embryos at 104 and 120 hpf are shown (D, H). A Student′s t-test was conducted to compare immature goblet cell numbers in agr2 morphants with those in wild type or agr2–5 mmMO1 and 5 mmMO2-coinjected embryos. *p<0.001. Scale bars represent 100 μm.