ZFIN Image: Camp et al., 2012, Fig. S4

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Figure Caption

Fig. S4

The zebrafish angptl4 in3.4 intestinal module exhibits hallmarks of a classical enhancer. (A) Dr in3.4 was cloned in an inverted orientation (in3.4(ds-iv)) downstream of GFP driven by -1 kb of the angptl4 promoter (Tg(-1angptl4:GFP:in3.4inv)). Mosaic and stable intestinal expression patterns are indistinguishable from those when in3.4 is upstream of the Fos minimal promoter (see Figure 3). The white arrow marks the boundary between the anterior intestine (segment 1) and mid-intestine (segment 2). The marked liver expression is likely conferred by the -1 kb angptl4 promoter (see Figure S3F). (B) The in3.2 module drives expression of a reporter (tdTomato) in the intestinal epithelium of adult zebrafish. (C) Nuclei were isolated from adult zebrafish epithelial cells and subjected to increasing concentrations of DNase I. Digested DNA from 0.5 units DNase I was used for quantitative PCR shown in Figure 3P.

Acknowledgments

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